Molecular cloning, expression analysis and sequence prediction of CCAAT/enhancer-binding protein beta gene of Qinchuan cattle

摘要

CCAAT/enhancer-binding protein?beta?(C/EBPβ), as?an essential transcriptional factor,?regulates?the differentiation of adipocytes?and the deposition of fat.?Herein,?we cloned the?whole open reading frame (ORF)?of?bovine?C/EBPβ gene?and?analyzed itsputative?protein structures?via DNA cloning and sequence analysis. Then, theexpression profile?of this?C/EBPβ gene?in fifteen kinds of tissues of?Qinchuan cattlewas conducted by?real-time polymerase chain reaction?(RT-PCR) technology. One significant?outside to inside?transmembrane?structure?located in?amino acid?region from?190?to?208 was observed in the putative protein sequences. Besides, one basic leucine zipper domain?(bZIP)?in amino acid?area?from 274?to 337 was found, concurring with the main characteristic of C/EBPs.?Homologous comparison?of the amino acid sequences from?C/EBPβ?cloned in this study and those from?different species indicated C/EBPβ?gene of Qinchuan?cattle?shared?97, 95 and 91% similarity with?Homo sapiens,?Sus scrofa?and?Oryctolagus?cuniculus?respectively, indicating a good?sequence?evolutional conservation of?C/EBPβ.?RT-PCR results revealed bovine C/EBPβ gene?mRNA expression?level?of subcutaneous fat was?the highest among all the analyzed?tissues, and the relative quantity (RQ) in fat tissue increased as cattle grew. All in all, the present results could be used as basic but important genetic resource and information to inspire additional and specific studies on Qinchuan cattle.