Rapid Differentiation of Pleurotus Ostreatus from Pleurotus Sapidus Using PCR Technique

摘要

Pleurotus ostreatus and Pleurotus sapidus are difficult to differentiate using standard morphologically based characteristics. This study, using two taxon-selective primers for the Internal Transcribed Spacer (ITS) region in the nuclear ribosomal repeat unit. These primers, ITS1-F & ITS4, were intended to be specific for the higher fungi, respectively. RAPD-PCR technique was used to reveal DNA polymorphism in DNA of the two fungi Pleurotus ostreatus and Pleurotus sapidus in order to search for the sources of differences that could be used as a DNA marker represent the differentiation between this two species. In RAPD-PCR, eight different decamer primers chosen randomly were employed to detect the genetic polymorphisms among studied strains.