Human HemK2/KMT9/N6AMT1 is an active protein methyltransferase, but does not act on DNA in vitro, in the presence of Trm112

摘要

Ever since the initial discovery and characterization ofS-adenosyl-L-methionine (SAM)1, the ATP-activated formof methionine, SAM has become the second most commonlyused enzyme cofactor after ATP. A wide variety ofmacromolecules, including DNA, RNA, proteins, polysaccharides,lipids, and a range of small molecules aresubject to methylation by highly specific SAM-dependentmethyltransferases (MTases) acting on a particular targetatom. Examples of methylation targets include nucleicacids (cytosine-C5, cytosine-N4 and adenine-N6), proteinresidues (arginine-N, lysine-N, glutamine-N and histidine-N),and small molecules (catechol-O, histamine-N,glycine-N, and thiopurine-S).