MicroRNA-182-5p protects human lens epithelial cells against oxidative stress-induced apoptosis by inhibiting NOX4 and p38 MAPK signalling

摘要

BACKGROUND:MicroRNAs (miRNAs) are abnormally expressed in various ocular diseases, including age-related cataract. However, the role of miR-182-5p in the progression of age-related cataract remains unclear.METHODS:The expression of miR-182-5p in HLE-B3 cells was detected by qRT-PCR. HLE-B3 cells were transfected with miR-182-5p mimics. CCK-8, EdU, flow cytometry, 2',7'-dichlorodihydrofluorescein diacetate, JC-1 kit, and western blot were used to assess the cell viability, proliferation, apoptosis, reactive oxygen species (ROS) level, mitochondrial membrane potential (MMP), and protein expression, respectively, in vitro. The relationship between miR-182-5p and NOX4 was confirmed using the dual-luciferase reporter gene analysis.RESULTS:We found that miR-182-5p expression was significantly decreased by the Hsub2/subOsub2/sub exposure. Overexpression of miR-182-5p promoted cell proliferation and inhibited ROS production and apoptosis in Hsub2/subOsub2/sub-induced HLE-B3 cells. Moreover, p-p-38, p-ERK, and p-JNK were up-regulated in Hsub2/subOsub2/sub-treated HLE-B3 cells, and overexpression of miR-182-5p reversed the effects of Hsub2/subOsub2/sub on HLE-B3 cells. In addition, dual-luciferase reporter assay substantiated that NOX4 was a direct target and downregulated by miR-182-5p.CONCLUSIONS:We concluded that miR-182-5p inhibited lens epithelial cells apoptosis through regulating NOX4 and p38 MAPK signaling, providing a novel biomarker for treatment of age-related cataract.