Integrated approach on azo dyes degradation using laccase enzyme and Cul nanoparticle

摘要

Azo dyes released by the textile industries cause severe damage to the environment and living organisms. The degradationof azo dyes is widely studied using enzymatic methods. Laccase is a copper-containing enzyme that degrades theazo dyes into less toxic compounds. In this work, the crude laccase enzyme produced by the alkaliphile Pseudomonasmendocina in the degradation of mixed azo dye showed 0.386 U/mL activity at pH 8.5. A combination of enzymatic andgreen synthesized nanoparticles was used in the degradation of mixed azo dye. Laccase used in the degradation of mixedazo dyes showed 58.4% in 72 h, while the photocatalytic degradation of mixed azo dyes showed 15.9%. The degradationof azo dyes using copper iodide nanoparticles resulted in 15.8% degradation. However, it was noticed that the combinedmethod of degradation of azo dyes involving both crude laccase and CuI nanoparticles gave a degradation of 62.3% in60 min. Interaction of laccase enzyme with azo dyes using in silico analysis predicted the binding energy with reactivered (−7.19 kcal/mol), reactive brown (−8.57 kcal/mol), and reactive black dyes (−9.17 kcal/mol) respectively.