The components of the proton motive force (delta p), namely the membrane potential and the transmembrane pH gradient, were measured in washed cells of Rhizobium japonicum CC705 grown in cultures (5% O2-95% N2) in the presence of 10 mM KNO3 and in bacteroids from Glycine max. The delta p and its components remained reasonably constant in cells as well as in bacteroids at various stages of growth. The effects of uncouplers and ATPase inhibitors on the delta p and its components were determined in both cultured cells and bacteroids. The data indicated that a respiration-driven H+ translocation is the source of the delta p in both cultured cells and bacteroids.
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机译：在存在10 mM KNO3的情况下，在生长于培养物（5％O2-95％N2）中的日本根瘤菌CC705洗涤过的细胞中，测量质子原动力的分量（δp），即膜电位和跨膜pH梯度。以及来自Glycine max。在生长的各个阶段，δp及其成分在细胞和类细菌中均保持合理的常数。在培养的细胞和类细菌中均测定了解偶联剂和ATPase抑制剂对Δp及其组分的影响。数据表明，呼吸驱动的H +易位是培养细胞和类细菌中δp的来源。
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