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首页> 外文期刊>Journal of Clinical Microbiology >Enzymatic profiles of Enterobacter sakazakii and related species with special reference to the alpha-glucosidase reaction and reproducibility of the test system.
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Enzymatic profiles of Enterobacter sakazakii and related species with special reference to the alpha-glucosidase reaction and reproducibility of the test system.

机译:阪崎肠杆菌及相关物种的酶学特征,尤其要参考α-葡萄糖苷酶反应和测试系统的可重复性。

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The enzymatic profiles of Enterobacter sakazakii, Enterobacter cloacae, Enterobacter aerogenes, and Enterobacter agglomerans were determined with the API ZYM system (API System S.A., La Balme Les Grottes, France). Each assay was performed three times. A simple formula was derived and applied to assess the reproducibility of the API ZYM tests. In addition, a separate alpha-glucosidase test was performed. All E. sakazakii isolates produced alpha-glucosidase, in contrast to the other Enterobacter isolates. No phosphoamidase activity was detected in any of the E. sakazakii isolates, whereas it was present in 72% of E. cloacae, 89% of E. agglomerans, and 100% of E. aerogenes isolates. It was concluded that detection of alpha-glucosidase permits rapid and reliable differentiation between E. sakazakii and other Enterobacter species. The reproducibilities of alpha-glucosidase and phosphoamidase reactions were estimated to be 89 and 81%, respectively.
机译:用API ZYM系统(API System S.A.,La Balme Les Grottes,France)确定了阪崎肠杆菌,阴沟肠杆菌,产气肠杆菌和团聚肠杆菌的酶谱。每个测定进行三次。导出了一个简单的公式,并将其用于评估API ZYM测试的可重复性。另外,进行了单独的α-葡萄糖苷酶测试。与其他肠杆菌分离株相比,所有阪崎肠杆菌分离株均产生α-葡萄糖苷酶。在阪崎肠杆菌的任何分离物中均未检测到磷酸酰胺酶活性,而在72%的阴沟肠杆菌,89%的聚集体大肠杆菌和100%的产气链球菌中均未检测到。结论是,α-葡萄糖苷酶的检测可以使阪崎肠杆菌和其他肠杆菌快速,可靠地区别开来。据估计,α-葡萄糖苷酶和磷酸酰胺酶反应的重现性分别为89%和81%。

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