SOX2 expression levels distinguish between neural progenitor populations of the developing dorsal telencephalon

摘要

TheHMG-BoxtranscriptionfactorSOX2isexpressedinneuralprogenitorpopulationsthroughoutthedevelopingandadultcentralnervoussystemandisnecessarytomaintaintheirprogenitoridentity.However,itisunclearwhetherSOX2levelsareuniformlyexpressedacrossallneuralprogenitorpopulations.Inthedevelopingdorsaltelencephalon,twodistinctpopulationsofneuralprogenitors,radialgliaandintermediateprogenitorcells,areresponsibleforgeneratingamajorityofexcitatoryneuronsfoundintheadultneocortex.Herewedemonstrate,usingbothcellularandmolecularanalyses,thatSOX2isdifferentiallyexpressedbetweenradialglialandintermediateprogenitorpopulations.Moreover,utilizingaSOX2supEGFP/supmouseline,weshowthatthisdifferentialexpressioncanbeusedtoprospectivelyisolatedistinct,viablepopulationsofradialgliaandintermediatecellsforeminvitro/emanalysis.Giventhelimitedrepertoireofcell-surfacemarkerscurrentlyavailableforneuralprogenitorcells,thisprovidesaninvaluabletoolforprospectivelyidentifyingandisolatingdistinctclassesofneuralprogenitorcellsfromthecentralnervoussystem./p/div