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Transcription Factor AP-2 Is an Essential and Direct Regulator of Epidermal Development in Xenopus

机译:转录因子AP-2是非洲爪蟾表皮发育必不可少的直接调节器。

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ExpressionoftheemXenopus/emhomologofthemammaliantranscriptionfactorAP-2α(XAP-2)isactivatedthroughouttheanimalhemisphereshortlyafterthemidblastulatransition,andbecomesrestrictedtoprospectiveepidermisbytheendofgastrulation,underthecontrolofBMPsignalmodulation.Elevatedexpressioninthefutureneuralcrestregionbeginsatthistime.EctopicexpressionofXAP-2canrestoretranscriptionofepidermalgenesinneuralizedectoderm,bothinectodermalexplantsandintheintactembryo.Likewise,lossofXAP-2function,accomplishedbyinjectionofantisenseoligonucleotidesorbyoverexpressionofantimorphicXAP-2derivatives,leadstolossofepidermalandgainofneuralgeneexpression.Thesetreatmentsalsoresultingastrulationfailure.Thus,AP-2isacriticalregulatorofectodermaldeterminationthatisrequiredfornormalepidermaldevelopmentandmorphogenesisinthefrogembryo./p/div
机译:Expressionofthe 爪蟾 homologofthemammaliantranscriptionfactorAP-2α(XAP-2)isactivatedthroughouttheanimalhemisphereshortlyafterthemidblastulatransition,andbecomesrestrictedtoprospectiveepidermisbytheendofgastrulation,underthecontrolofBMPsignalmodulation.Elevatedexpressioninthefutureneuralcrestregionbeginsatthistime.EctopicexpressionofXAP-2canrestoretranscriptionofepidermalgenesinneuralizedectoderm,bothinectodermalexplantsandintheintactembryo.Likewise,lossofXAP-2function,accomplishedbyinjectionofantisenseoligonucleotidesorbyoverexpressionofantimorphicXAP-2derivatives,leadstolossofepidermalandgainofneuralgeneexpression.Thesetreatmentsalsoresultingastrulationfailure.Thus,AP-2isacriticalregulatorofectodermaldeterminationthatisrequiredfornormalepidermaldevelopmentandmorphogenesisinthefrogembryo

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