Enhancement of differentiation of lens and pigment cells by ascorbic acid in cultures of neural retinal cells of chick embryos

摘要

Whendissociatedcellsofneuralretinaeof9-day-oldchickembryoswereculturedinEagle'sminimumessentialmediumsupplementedwithdialyzedfetalcalfserum,boththeproliferationanddifferentiationoftheneuralretinalcellswereinhibited.Thesecellsremainedquiescentandflattened.Whenascorbicacidwasaddedtosuchamedium,thecellsstartedtogrowanddifferentiatedintolentoidbodiesandpigmentedcellsafterabout10days.