Targeted knock‐out of a gene encoding sulfite reductase in the moss Physcomitrella patens affects gametophytic and sporophytic development

摘要

Akeystepinsulfateassimilationintocysteineisthereductionofsulfitetosulfidebysulfitereductase(SiR).ThisenzymeisencodedbythreegenesinthemossPhyscomitrellapatens.Toobtainafirstinsightintotherolesoftheindividualisoforms,wedeletedthegeneencodingtheSiR1isoforminP.patensbyhomologousrecombinationandsubsequentlyanalysedtheΔSiR1mutants.WhileΔSiR1mutantsshowednoobviousalterationinsulfurmetabolism,theirregenerationfromprotoplastsandtheirabilitytoproducematuresporeswassignificantlyaffected,highlightinganunexpectedlinkbetweenmosssulfateassimilationanddevelopment,thatisyettobecharacterized...