Knock‐out of metacaspase and/or cytochrome c results in the activation of a ROS‐independent acetic acid‐induced programmed cell death pathway in yeast

摘要

Togainfurtherinsightintoyeastaceticacid‐inducedprogrammedcelldeath(AA‐PCD)weanalyzedtheeffectsoftheantioxidantN‐acetyl‐l‐cysteine(NAC)oncellviability,hydrogenperoxide(H2O2)production,DNAfragmentation,cytochromec(cytc)releaseandcaspase‐likeactivationinwildtype(wt)andmetacaspaseand/orcytc‐lackingcells.WefoundthatNACpreventsAA‐PCDinwtcells,byscavengingH2O2andbyinhibitingbothcytcreleaseandcaspase‐likeactivation.Thisshowstheoccurrenceofareactiveoxygenspecies(ROS)‐dependentAA‐PCD.ContrarilynoNACdependentchangeinAA‐PCDofmutantcellswasdetectable,showingthataROS‐independentAA‐PCDcanalsooccur...