Yeast acetic acid‐induced programmed cell death can occur without cytochrome c release which requires metacaspase YCA1

摘要

Toinvestigatetheroleofcytochromec(cytc)releaseinyeastaceticacid‐inducedprogrammedcelldeath(AA‐PCD),wildtype(wt)andcellslackingmetacaspase(Δyca1),cytochromec(Δcyc1,7)andboth(Δcyc1,7Δyca1)werecomparedforAA‐PCDoccurrence,hydrogenperoxide(H2O2)productionandcaspaseactivity.AA‐PCDoccursinΔcyc1,7andΔcyc1,7Δyca1cellsslowerthaninwt,butsimilartothatinΔyca1cells,inwhichnocytochromecreleaseoccurs.BothH2O2productionandcaspaseactivationoccurinthesecellswithearlyandextra‐activationinΔcyc1,7cells.WeconcludethatalternativedeathpathwayscanbeactivatedinyeastAA‐PCD,onedependentoncytcrelease,whichrequiresYCA1,andtheother(s)independentonit...