Tyrosine partners coordinate DNA nicking by the Salmonella typhimurium plasmid pCU1 relaxase enzyme

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Tyrosine partners coordinate DNA nicking by the Salmonella typhimurium plasmid pCU1 relaxase enzyme

机译：酪氨酸伴侣通过鼠伤寒沙门氏菌质粒pCU1松弛酶协调DNA切割

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Conjugativeplasmidtransferresultsinthespreadofantibioticresistancegenesandvirulencefactorsbetweenbacterialcells.PlasmidtransferisdependentupontheDNAnickingactivityofaplasmid‐encodedrelaxaseenzyme.TyrosineresidueswithintherelaxasecleavetheDNAplasmidnicsiteinahighlysequence‐specificmanner.TheconjugativeresistanceplasmidpCU1encodesarelaxasewithfourtyrosineresiduessurroundingitsactivesite(Y18,19,26,27).WeuseactivityassaystodemonstratethatthepCU1relaxasepreferentiallyusesY26oracombinationofY18+19tonickDNAatwildtypelevels,andthatanadjacentasparticaciddeprotonatesthesetyrosinestoactivatethemforattack.OurfindingsillustratetheuniquemodificationsthatthepCU1relaxasehasintroducedintothetraditionalrelaxase‐mediatedDNAnickingmechanism...

机译：Conjugativeplasmidtransferresultsinthespreadofantibioticresistancegenesandvirulencefactorsbetweenbacterialcells.PlasmidtransferisdependentupontheDNAnickingactivityofaplasmid-encodedrelaxaseenzyme.TyrosineresidueswithintherelaxasecleavetheDNAplasmidnicsiteinahighlysequence-specificmanner.TheconjugativeresistanceplasmidpCU1encodesarelaxasewithfourtyrosineresiduessurroundingitsactivesite（Y18,19,26,27）.WeuseactivityassaystodemonstratethatthepCU1relaxasepreferentiallyusesY26oracombinationofY18 + 19tonickDNAatwildtypelevels，andthatanadjacentasparticaciddeprotonatesthesetyrosinestoactivatethemforattack.OurfindingsillustratetheuniquemodificationsthatthepCU1relaxasehasintroducedintothetraditionalrelaxase-mediatedDNAnickingmechanism ...

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《FEBS Letters 》 |2011年第8期| 共7页
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1. Tyrosine partners coordinate DNA nicking by the Salmonella typhimurium plasmid pCU1 relaxase enzyme. [J] . Nash RP, Niblock FC, Redinbo MR FEBS letters. . 2011 ,第8期

机译：酪氨酸伴侣通过鼠伤寒沙门氏菌质粒pCU1松弛酶来协调DNA切割。
2. The mechanism and control of DNA transfer by the conjugative relaxase of resistance plasmid pCU1 [J] . Matthew R. Redinbo, Rebekah Potts Nash, Scott A. Lujan, Nucleic acids research . 2010 ,第17期

机译：抗性质粒pCU1的结合松弛酶介导DNA转移的机制和控制
3. Unique Helicase Determinants in the Essential Conjugative TraI Factor from Salmonella enterica Serovar Typhimurium Plasmid pCU1 [J] . Krystle J. McLaughlin, Rebekah P. Nash, Mathew R. Redinbo Journal of bacteriology . 2014 ,第17期

机译：肠炎沙门氏菌鼠伤寒沙门氏菌质粒pCU1的必需结合TraI因子中的独特解旋酶决定因素。
4. Kinetic of Tissue Metabolism after Oral Administration of Recombinant Attenuated Salmonella Typhimurium Carrying Plasmid pcDNA3s in Mice [C] . WANG Xue-qing, rnBING Xing-guo, rnLI Hai-li Asian Pacific Confederation of Chemical Engineering congress . 2008

机译：重组减毒鼠伤寒沙门氏菌携带质粒pcDNA3s口服后小鼠的组织代谢动力学。
5. Biological and virulence characteristics of Salmonella enterica serovar Typhimurium following deletion of genes encoding the tRNA modification enzymes GidA and MnmE. [D] . Shippy, Daniel C. 2013

机译：删除编码tRNA修饰酶GidA和MnmE的基因后，肠炎沙门氏菌鼠伤寒沙门氏菌的生物学和毒力特征。
6. Tyrosine Partners Coordinate DNA Nicking by the Salmonella typhimurium Plasmid pCU1 Relaxase Enzyme [O] . Rebekah P. Nash, Franklin C. Niblock, Matthew R. Redinbo -1

机译：酪氨酸合作伙伴协调DNa切口由鼠伤寒沙门菌质粒pCU1 Relaxase酶
7. Tyrosine partners coordinate DNA nicking by the Salmonella typhimurium plasmid pCU1 relaxase enzyme [O] . Nash Rebekah P., Niblock Franklin C., Redinbo Matthew R. 2011

机译：酪氨酸伴侣通过鼠伤寒沙门氏菌质粒pCU1松弛酶协调DNA切割
8. Novel Virulence Properties of the Salmonella typhimurium Virulence-Associated Plasmid: Immune Suppression and Stimulation of Splenomegaly. [R] . Hoertt, B. E., Ou, J., Kopecko, D. J., 1989

机译：鼠伤寒沙门氏菌毒力相关质粒的新毒力特性：脾脏肿大的免疫抑制和刺激。

Tyrosine partners coordinate DNA nicking by the Salmonella typhimurium plasmid pCU1 relaxase enzyme

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