Comparative genome‐scale metabolic modeling of actinomycetes: The topology of essential core metabolism

摘要

Actinomycetesarehighlyimportantbacteria.Ononehand,someofthemcauseseverehumanandplantdiseases,ontheotherhand,manyspeciesareknownfortheirabilitytoproduceantibiotics.Herewereporttheresultsofacomparativeanalysisofgenome‐scalemetabolicmodelsof37speciesofactinomycetes.Basedoninsilicoknockoutswegeneratedtopologicalandgenomicmapsforeachorganism.Combiningthecollectionofgenome‐widemodels,weconstructedaglobalenzymeassociationnetworktoidentifybothaconserved“corenetwork”andan“essentialcorenetwork”oftheentiregroup.Ashasbeenreportedforlow‐degreemetabolitesinseveralorganisms,low‐degreeenzymes(inlinearpathways)turnouttobegenerallymoreessentialthanhigh‐degreeenzymes(inmetabolichubs)...