The endogenous siRNA pathway in Drosophila impacts stress resistance and lifespan by regulating metabolic homeostasis

摘要

Smallnon‐codingRNAsregulategeneexpressioninasequence‐specificmanner.InDrosophila,Dicer‐2(Dcr‐2)functionsinthebiogenesisofendogenoussmallinterferingRNAs(endo‐siRNAs).Weidentified21distinctproteinsthatexhibiteda?1.5‐foldchangeasaconsequenceoflossofdcr‐2function.Mostoftheseweremetabolicgenesimplicatedinstressresistanceandaging.dcr‐2Mutantshadreducedlifespanandwerehypersensitivetooxidative,endoplasmicreticulum,starvation,andcoldstresses.Furthermore,lossofdcr‐2functionledtoabnormallipidandcarbohydratemetabolism.Ourresultssuggestrolesfortheendo‐siRNApathwayinmetabolicregulationanddefenseagainststressandaginginDrosophila...