Bafilomycin A1 induces apoptosis in PC12 cells independently of intracellular pH

摘要

>PC12 cells growth-arrested with bafilomycin A₁ died showing apoptotic chromatin condensation in the nuclei. The bafilomycin A₁-induced chromatin condensation was preceded by neurite outgrowth (NOG), required higher concentrations of bafilomycin A₁ than NOG, and was suppressed by cycloheximide and aurintricarboxylic acid. NH₄Cl (10 mM), another acidotropic pH perturbing agent, neither induced apoptotic chromatin condensation by itself nor suppressed that induced by bafilomycin A₁, suggesting that bafilomycin A₁-induced apoptosis occurs independently of intracellular pH in PC12 cells.