Reconstitution of photosynthetic oxygen evolving activity by rebinding of 33 kDa protein to CaCl2‐extracted PS II particles

摘要

>On extracting the O₂-evolving Photosystem II (PS II) particles with CaCl₂ solution, three proteins of 33, 24 and 16 kDa were completely liberated from the particles concomitant with total inactivation of O₂ evolution, but most of the Mn atoms present in the initial particles were preserved unaffected being still associated with the particles. On reading the extracted proteins to the extracted particles, a considerable amount of 33 kDa protein rebound to the particles concomitant with significant reconstitution of O₂-evolving activity.