Reconstitution of photosynthetic charge accumulation and oxygen evolution in CaCl2‐treated PS II particles

摘要

>Extraction of PS II particles with 1 M CaCl₂ caused complete disappearance of the light-induced signal of the possible Kok S₂ state of the water-splitting complex and total loss of the O₂, evolving activity, concomitant with perfect removal of the 17-, 23- and 34-kDa proteins from the particles. The recovery of the multiline signal in the CaCl₂-treated PS II was performed by reinserting the 34-kDa protein, when CI− was present in the solution for the EPR measurement. However, in the absence of Cl−, besides the 34-kDa protein, the 17- and 23-kDa proteins were required for the recovery of the signal. These results are compared with the results on the recovery of the O₂, evolution in the reconstituted PS II to examine the role of these three proteins on the water splitting.