>We have detected unique nucleoprotein particles specific for the 18–28 S rDNA nontranscribed spacer of Drosophilamelanogaster. The particles migrate between di- and trinucleosomes on nucleoprotein gels, and are between mono- and dinucleosomal in DNA length. These migration properties suggest that the nontranscribed spacer particles could have a protein component larger than a histone core. The variant nucleoprotein structures map primarily within the nontranscribed spacer 235 base pair internal subrepeat, which is AT-rich and possesses a 50 base pair sequence homologous to the RNA polymerase I binding site.
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机译:>我们检测到了特有果蝇 em> 黑猩猩 em>的18–28 S rDNA非转录间隔子的独特核蛋白颗粒。颗粒在核蛋白凝胶上的二核小体和三核小体之间迁移,DNA长度在单核小体和二核小体之间。这些迁移特性表明,非转录间隔子颗粒的蛋白质成分可能大于组蛋白核心。变体核蛋白结构主要在非转录间隔区235个碱基对的内部亚重复序列中定位,该区域是富含AT的,并具有与RNA聚合酶I结合位点同源的50个碱基对的序列。
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