DNA replication and UV-induced DNA repair synthesis in human fibroblasts are much less sensitive than DNA polymerase α to inhibition by butylphenyl-deoxyguanosine triphosphate

摘要

In mammalian cells, both semiconservative DNA replication and the DNA repair patch synthesis induced by high doses of ultraviolet radiation are known to be inhibited by aphidicolin, Indicating the involvement in these processes of one or both of the aphidicolin-sensitive DNA polymerases. α and/or δ. In this paper, N2-(p-n-butylphenyl)-2′ -deoxyguanosine- 5′-triphosphate. a strong inhibitor of polymerase α and a weak inhibitor of polymerase δ, is used to further characterize the DNA polymerase(s) involved in these two forms of nuclear DNA synthesis. In permeable human fibroblasts, DNA replication and ultraviolet-induced DNA repair synthesis are more resistant to the inhibitor than DNA polymerase α by factors of approximately 500 and 3000, respectively. These findings are most consistent with the involvement of DNA polymerase δ in these processes.