Nudeotide sequence of the transforming early region Elb of adenovirus type 12 DNA: structure and gene organization, and comparison with those of adenovirus type 5 DNA

摘要

The nudeotide sequence of the entire transforming early region Elb of the highly oncogenic adenovirus type 12 (Adl2) DNA has been determined. The total sequence (3860 base pairs) encompasses the entire transforming early region El of Adl2 DNA. From the sequence for the Elb region of Adl2, and the transcription map of the Elb region (1, 2, 3, and this paper) the structure and gene organization of the early region Elb of Adl2 DNA were analyzed and compared with those of the Elb region in the non-oncogenic Ad5 DNA (4, 5). Most of the sequences in the Elb region of Adl2 was highly homologous to that of Ad5. It is predicted that the Adl2 region Elb codes for polypeptides of 53.9, 19.1, and 8.9 kd. This situation is identical with that of the Ad5 region Elb which codes for polypeptides of 54.9, 20.6, and 8.3 kd. The function of these predicted polypeptides encoded by the Elb regions in cell transformation is discussed.