...
首页> 外文期刊>Molecular and Cellular Biology >Actions of Rho family small G proteins and p21-activated protein kinases on mitogen-activated protein kinase family members.
【24h】

Actions of Rho family small G proteins and p21-activated protein kinases on mitogen-activated protein kinase family members.

机译:Rho家族小G蛋白和p21激活的蛋白激酶对有丝分裂原激活的蛋白激酶家族成员的作用。

获取原文
   

获取外文期刊封面封底 >>

       

摘要

The mitogen-activated protein (MAP) kinases are a family of serine/threonine kinases that are regulated by distinct extracellular stimuli. The currently known members include extracellular signal-regulated protein kinase 1 (ERK1), ERK2, the c-Jun N-terminal kinase/stress-activated protein kinases (JNK/SAPKs), and p38 MAP kinases. We find that overexpression of the Ste20-related enzymes p21-activated kinase 1 (PAK1) and PAK2 in 293 cells is sufficient to activate JNK/SAPK and to a lesser extent p38 MAP kinase but not ERK2. Rat MAP/ERK kinase kinase 1 can stimulate the activity of each of these MAP kinases. Although neither activated Rac nor the PAKs stimulate ERK2 activity, overexpression of either dominant negative Rac2 or the N-terminal regulatory domain of PAK1 inhibits Ras-mediated activation of ERK2, suggesting a permissive role for Rac in the control of the ERK pathway. Furthermore, constitutively active Rac2, Cdc42hs, and RhoA synergize with an activated form of Raf to increase ERK2 activity. These findings reveal a previously unrecognized connection between Rho family small G proteins and the ERK pathway.
机译:丝裂原激活蛋白(MAP)激酶是丝氨酸/苏氨酸激酶家族,受不同的细胞外刺激调节。目前已知的成员包括细胞外信号调节蛋白激酶1(ERK1),ERK2,c-Jun N端激酶/应激激活蛋白激酶(JNK / SAPKs)和p38 MAP激酶。我们发现,Ste20相关酶p21激活的激酶1(PAK1)和PAK2在293细胞中的过量表达足以激活JNK / SAPK,并在较小程度上激活了p38 MAP激酶,但不足以激活ERK2。大鼠MAP / ERK激酶激酶1可刺激每种MAP激酶的活性。尽管激活的Rac或PAK均未刺激ERK2活性,但显性负性Rac2或PAK1的N端调节域的过度表达均抑制了Ras介导的ERK2激活,表明Rac在ERK途径控制中的许可作用。此外,组成型活性Rac2,Cdc42hs和RhoA与激活形式的Raf协同作用以增加ERK2活性。这些发现揭示了Rho家族小G蛋白与ERK途径之间以前未被认识的联系。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号