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首页> 外文期刊>Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation >MONOSOMIC ANALYSIS OF FATTY ACID COMPOSITION IN EMBRYO LIPIDS OF ZEA MAYS L
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MONOSOMIC ANALYSIS OF FATTY ACID COMPOSITION IN EMBRYO LIPIDS OF ZEA MAYS L

机译:玉米胚芽脂中脂类脂肪酸组成的单分子分析

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摘要

The effects of monosomy of specific chromosomes on the fatty acid composition of maize embryos were studied. A novel technique was developed to obtain fatty acid profiles of single embryos without reducing the viability of the sampled kernels. Monosomic 2 embryos had significantly more oleic acid and significantly less linoleic acid than diploid control embryos. Since the conversion of oleic acid to linoleic acid is a single-enzyme-mediated reaction, we suggest that a gene involved in linoleic acid biosynthesis is located on chromosome 2. Additional consistent variations were found in other monosomic types. This study demonstrates that monosomic analysis can be used to study gene dosage effects at the biochemical level.
机译:研究了特定染色体的单体性对玉米胚脂肪酸组成的影响。开发了一种新技术来获得单个胚胎的脂肪酸谱,而又不降低所采样果仁的活力。 Monosomic 2胚胎比二倍体对照胚胎具有更多的油酸和更少的亚油酸。由于油酸向亚油酸的转化是单酶介导的反应,因此我们建议涉及亚油酸生物合成的基因位于2号染色体上。在其他单体型中还发现了其他一致的变异。这项研究表明,单体分析可以用于在生化水平研究基因剂量效应。

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