We applied multispacer typing (MST) by incorporating 9 variable intergenic spacers to Bartonella henselae DNA detected in lymph node biopsy specimens from 70 patients with cat-scratch disease (CSD), in cardiac valve specimens from 2 patients with endocarditis, and in 3 human isolates from patients with bacillary angiomatosis, CSD, and endo-carditis. Sixteen MST genotypes were found, 5 previously identifi ed in cats and 11 new. Of the studied DNA, 78.7% belonged to 2 genotypes, which were phylogenetically orga-nized into 4 lineages. Human strains were mostly grouped within 2 lineages, previously identifi ed as Marseille and Houston-1. Our results suggest a greater genetic diversity in human-infecting B. henselae than what has previously been evaluated by using other genotyping methods. How-ever, the diversity is not signifi cantly different from that of cat strains. MST is thus a suitable genotyping tool for evalu-ating the genetic heterogeneity of B. henselae among iso-lates obtained from human patients
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