Rotavirus diarrhea in Kashmir: Detection of genotype G12P[6] strains

摘要

Background: Rotavirus genotype G12P[6] was detected among children with acute gastroenteritis in Kashmir Valley-India. The study was conducted in two major hospitals of the Valley from Jan 2006 to Jan 2007. Six (6) cases of G12 infection were identified having P[6] specifity. Three (3) cases of G12 P[6] were detected by PCR and other three (3) by gene sequencing. Sequence analysis of the VP7 gene from three neonatal strains indicated a high level of aminoacid homology (98-99%) to other G12 strains reported world wide, suggesting introduction of novel rotavirus strain serotype into the community.The G12 strains were detected sporadically and were not associated with any outbreak or nosocomial infection.Genetic characterization of VP7 gene of these strains revealed one lineage and a very little aminoacid divergence <=2%.