Amplification of pre-membrane and non-structural region 5 for detection and typification of Dengue virus by real-time PCR using SYBR green I assay

摘要

Background: Confirmation of Dengue virus [DV] infection by laboratory and serotyping is critical information for patient's management and to applying public health strategies to control the Aedes aegyptis vector. Molecular biology methods have largely displaced the classical cell culture techniques reducing the time of reporting. The aim of this study was standardized a Real-Time PCR assay for detection, serotypification and quatification of the DV using SYBR? green I RT-PCR technologies.