Antigenic differences between Parastrongylus cantonensis and Parastrongylus malaysiensis revealed by a monoclonal antibody

摘要

Objective: To confirm antigenic differences between Parastrongylus cantonensis and Parastrongylus malaysiensis.Methods: Sodium dodecyl sulfate- polyacrylamide gel electrophoresis and immunoblotting were conducted.Results: Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the somatic extracts of P. cantonensis and P. malaysiensis adult worms were highly complex with no apparent differences in the gel patterns between the two species. Immunoblot analysis using AW-3C2, a monoclonal antibody produced against the adult worm of P. cantonensis, showed variable staining smears in Parastrongylus adult worm antigens. With male worm antigens, a distinct antigenic band with apparent molecular weight of 205 kDa in P. cantonensis and one of 180 kDa in P. malaysiensis were observed.Conclusion: Antigenic differences exist between P. cantonensis and P. malaysiensis among proteins with apparently identical molecular weight.