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Use of an anti-human leukocyte interferon monoclonal antibody for the purification and radioimmunoassay of human alpha interferon.

机译:抗人白细胞干扰素单克隆抗体在人α干扰素的纯化和放射免疫测定中的用途。

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Mouse monoclonal antibody directed against human leukocyte alpha interferon (IFN-alpha) was coupled to Sepharose and used as an immunoadsorbent to purify human IFN-alpha. Leukocyte and lymphoblastoid (Namalva) IFNs were retained by the immunoadsorbent with a specificity of 80 to 100% and 40 to 60%, respectively. Human IFN-beta or -gamma and mouse IFN were not retained. The purified IFN-alpha retained its antiviral and anticellular properties as well as its ability to induce the 2-5A synthetase in human cells with a specific activity similar to that of the crude IFN. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of radioactively labeled IFN showed that it consisted of several proteins in the molecular weight range of 17,000 to 27,000. 125I-labeled IFN-alpha with a high specific activity (2,000 Ci/mmol) was used in a radioimmunoassay for the titration of IFN-alpha.
机译:将针对人白细胞α干扰素(IFN-α)的小鼠单克隆抗体与Sepharose偶联,用作纯化人IFN-α的免疫吸附剂。免疫吸附剂保留了白细胞和淋巴母细胞(Namalva)IFN,特异性分别为80%至100%和40%至60%。人IFN-β或-γ和小鼠IFN未保留。纯化的IFN-α保留了其抗病毒和抗细胞特性,以及诱导人细胞中2-5A合成酶的能力,具有与粗制IFN相似的比活性。放射性标记干扰素的十二烷基硫酸钠-聚丙烯酰胺钠凝胶电泳显示,它由分子量范围为17,000至27,000的几种蛋白质组成。具有高比活性(2,000 Ci / mmol)的125 I标记的IFN-α用于放射免疫分析中,用于IFN-α的滴定。

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