A Comparative Study in Using Solid Phase Assays as Technics in a Screening of Anti-HLA Antibodies

摘要

Objectives: Introduction of solid phase assays for HLA antibodies detection led to application of new algorithms for the immunological monitoring of patients in renal transplantation and prevented many cases of hum oral rejection. Methods: In this work, we made a single observation of a comparative study between ELISA and LUMINEX ? on 60 patient’s sera prior to start using in routine LUMINEX ? at the two compatibility department of Pasteur Institute Casablanca, Morocco. Data were analyzed following manufacturer recommendations. Finding: Concordance rates between the results for individual HLA Class I and Class II antigens measured by both techniques were found to be 73, 3%. Whereas discrepancies were around 25%. The use of sensitive and specific techniques in transplantation is an important parameter which could improve this field. Improvement: Following this study, significant difference between the tests was found, however LUMINEX detect HLA antibodies with higher sensitivity, which can provide additional information for the graft outcome.