Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant

Harold J. Schreier; Wipawee Dejtisakdi; Jose Ortega Escalante; Marina Brailo

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Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant

机译：嗜乳酸菌的转座子诱变和pckA突变体的分析

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A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA , encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluconeogenic role for pckA in P. limnophilus .

机译：发现使用EZ-Tn5转座体产生的嗜热链霉菌突变体在pckA内具有一个插入片段，编码磷酸烯醇丙酮酸羧化激酶。 pckA表达的破坏和酶活性的消除导致在无葡萄糖培养基中生长不良，这证明了pckA在嗜睡疟原虫中的糖原异生作用。

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《Applied Microbiology》 |2012年第19期|共4页
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Harold J. Schreier; Wipawee Dejtisakdi; Jose Ortega Escalante; Marina Brailo;
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1. Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant [J] . Harold J. Schreier, Wipawee Dejtisakdi, Jose Ortega Escalante, Applied and Environmental Microbiology . 2012,第19期

机译：嗜乳酸菌的转座子诱变和pckA突变体的分析
2. Transposon Mutagenesis of Azospirillum brasilense and Azospirillum lipoferum: Physical Analysis of Tn5 and Tn5-Mob Insertion Mutants [J] . Mark Vanstockem, Kris Michiels, Jos Vanderleyden, Applied and Environmental Microbiology . 1987,第2期

机译：巴西固氮螺菌和脂铁螺旋螺菌的转座子诱变：Tn5和Tn5-Mob插入突变体的物理分析
3. Transposon Express, a software application to report the identity of insertions obtained by comprehensive transposon mutagenesis of sequenced genomes: analysis of the preference for in vitro Tn5 transposition into GC-rich DNA - art. no. e113 [J] . Herron PR, Hughes G, Chandra G, Nucleic Acids Research . 2004,第14期

机译：Transposon Express，一种软件应用程序，用于报告通过测序基因组的全面转座子诱变获得的插入物的身份：分析将体外Tn5换位为富含GC的DNA的偏好-技术。没有。 e113
4. Isolation of Magnetospirillum magneticum AMB-1 Mutants Defective in Bacterial Magnetic Particle Synthesis by Transposon Mutagenesis [C] . TADASHI MATSUNAGA, HARUKO TAKEYAMA, ARIS TRI WAHYUDI Symposium on biotechnology for fuels and chemicals . 2001

机译：通过转座诱变的细菌磁性颗粒合成磁截面的隔离型磁孢子突变体
5. Design of a bioinformatics system for insertional mutagenesis analysis and its application to the sleeping beauty transposon system. [D] . Nannapaneni, Kishore. 2011

机译：用于插入诱变分析的生物信息学系统的设计及其在睡眠美容转座子系统中的应用。
6. Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant [O] . Harold J. Schreier, Wipawee Dejtisakdi, Jose Ortega Escalante, 2012

机译：嗜线菌的转座子诱变和pckA突变体的分析
7. Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant [O] . Harold J. Schreier, Wipawee Dejtisakdi, Jose Ortega Escalante, 2012

机译：Panctomyces Limnophilus的转座诱变和Pcka突变体的分析
8. Microarray Analysis of Transposon Insertion Mutants in Bacillus Anthracis: Global Identification of Genes Required for Sporulation and Germination [R] . Day, J. W. , Rasmussen, S. L. , Carpenter, B. M. , 2007

机译：炭疽杆菌转座子插入突变体的微阵列分析：孢子形成和萌发所需基因的全局鉴定

Transposon Mutagenesis of Planctomyces limnophilus and Analysis of a pckA Mutant

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