Biochemical Characterization of a Novel Haloalkane Dehalogenase from a Cold-Adapted Bacterium

Ivana Drienovska; Eva Chovancova; Tana Koudelakova; Jiri Damborsky; Radka Chaloupkova

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Biochemical Characterization of a Novel Haloalkane Dehalogenase from a Cold-Adapted Bacterium

机译：从冷适应细菌的新型卤代烷脱卤酶的生化特性。

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A haloalkane dehalogenase, DpcA, from Psychrobacter cryohalolentis K5, representing a novel psychrophilic member of the haloalkane dehalogenase family, was identified and biochemically characterized. DpcA exhibited a unique temperature profile with exceptionally high activities at low temperatures. The psychrophilic properties of DpcA make this enzyme promising for various environmental applications.

机译：鉴定并生化鉴定了来自Psychrobacter cryohalolentis K5的卤代烷脱卤酶DpcA，代表卤代烷脱卤酶家族的新型嗜冷成员。 DpcA表现出独特的温度曲线，在低温下具有异常高的活性。 DpcA的嗜冷特性使该酶有望用于各种环境应用。

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《Applied Microbiology》 |2012年第14期|共4页
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Ivana Drienovska; Eva Chovancova; Tana Koudelakova; Jiri Damborsky; Radka Chaloupkova;
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1. Biochemical Characterization of a Novel Haloalkane Dehalogenase from a Cold-Adapted Bacterium [J] . Ivana Drienovska, Eva Chovancova, Tana Koudelakova, Applied Microbiology . 2012,第14期

机译：从冷适应细菌的新型卤代烷脱卤酶的生化特性。
2. Construction and characterization of histidine-tagged haloalkane dehalogenase (LinB) of a new substrate class from a gamma-hexachlorocyclohexane-degrading bacterium, Sphingomonas paucimobilis UT26. [J] . Nagata Y, Damborsky J, Takagi M, Protein Expression and Purification . 1999,第2期

机译：组氨酸标记的卤代烷脱卤酶（LinB）的构建和表征，该新底物来自γ-六氯环己烷降解细菌鞘氨醇单胞菌UT26。
3. Purification and characterization of a haloalkane dehalogenase of a new substrate class from a gamma-hexachlorocyclohexane-degrading bacterium, Sphingomonas paucimobilis UT26. [J] . Y Nagata, K Miyauchi, J Damborsky, Applied and Environmental Microbiology . 1997,第9期

机译：γ-六氯环己烷降解细菌Sphingomonas paucimobilis UT26的新底物类卤代烷脱卤酶的纯化和表征。
4. ENGINEERING OF HALOALKANE DEHALOGENASE ENANTIOSELECTIVITY TOWARDS B-BROMOALKANES: OPEN-SOLVATED VERSUS OCCLUDED-DESOLVATED ACTIVE SITES [C] . Radka Chaloupkova, Veronika Liskova, Veronika Stepankova, Enzyme Engineering Conference . 2018

机译：卤代烷烃脱氢酶对B-溴烷烃的映致映选择性的工程：开放式溶剂与闭塞式去溶剂活性位点
5. Functional Genomic, Microbiological and Biochemical Characterization of Plant Biomass Deconstruction by the Extrememly Thermophilic Bacterium Caldicellulosiruptor saccharolyticus. [D] . VanFossen, Amy Lynne. 2010

机译：极端嗜热细菌Caldicellulosiruptor saccharolyticus对植物生物量解构的功能基因组学，微生物学和生化特征。
6. Biochemical Characterization of a Novel Haloalkane Dehalogenase from a Cold-Adapted Bacterium [O] . Ivana Drienovska, Eva Chovancova, Tana Koudelakova, 2012

机译：从冷适应细菌的新型卤代烷脱卤酶的生化特性。
7. Biochemical characterization of two haloalkane dehalogenases: DccA from C aulobacter crescentus and DsaA from S accharomonospora azurea [O] . Lauren Carlucci, Edward Zhou, Vladimir N. Malashkevich, 2016

机译：两种卤代烷烃脱氢酶的生化特征：来自C A1CRONONOSPORA AZUREA的C Aulobacter Crescentus和Dsaa的DCCA

Biochemical Characterization of a Novel Haloalkane Dehalogenase from a Cold-Adapted Bacterium

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