A strain identified as Corynebacterium fascians was found to accumulate extracellular D-alanine from glycerol. Cultural conditions for the accumulation of D-alanine were investigated and, as a result, a yield of 7 g of D-alanine per liter was obtained after a 96-h incubation in a medium containing 5% glycerol, 4% (NH4)2HPO4, and 0.3% corn steep liquor. Optical purity of D-alanine was dependent upon the concentration of corn steep liquor. At the optimal condition, almost optically pure D-alanine was formed and readily isolated (5 g/liter) from the fermentation broth. The product was not contaminated with any detectable amount of other amino acids, except for glycine which was present at a concentration of less than 1 percent.
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机译：发现被鉴定为法西氏棒杆菌的菌株从甘油中积累细胞外D-丙氨酸。研究了D-丙氨酸积累的培养条件，结果在含有5％甘油，4％（NH4）的培养基中孵育96小时后，每升D-丙氨酸的产量为7 g ）2HPO4和0.3％的玉米浆。 D-丙氨酸的光学纯度取决于玉米浆的浓度。在最佳条件下，几乎形成了光学纯的D-丙氨酸，并很容易从发酵液中分离出来（5克/升）。除了甘氨酸的含量少于1％以外，产品没有被任何可检测量的其他氨基酸污染。
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