Liquid Scintillation Radioassay in Disposable Microcentrifuge Tubes: Radioimmune Precipitates and Other Applications

摘要

A simple, economical radioassay system employing disposable polypropylene microcentrifuge tubes was developed. Plastic adapters permitted automatic operation in liquid scintillation spectrometers. Counting efficiencies of 3H, 14C, 32P, and 125I in liquid scintillation cocktails and of 32P by Cerenkov radiation (at lower efficiency in absence of added scintillator) were comparable to those in standard vials. Multipurpose use of the microtubes made the system versatile and expedient, e.g., collection of precipitates and radioassay in the same container. Collection of radioimmune precipitates was aided by a carrier inorganic precipitate, Mg2P2O7.