High-titered yields of human cytomegalovirus (CMV), strain AD 169, were produced in WI-38 cells in large roller bottles. Maximum plaque titers were observed by the 4th day after infection at which time infectivity in the medium was 200 times greater than that associated with the cells. Virus released into the medium was recovered by sedimentation in a sucrose gradient in a continuous-flow centrifuge rotor. Maximal viral infectivity was found at a sucrose concentration of 42%, equivalent to a density of 1.18 g/cm3. Deoxyribonucleic acid extracted from these preparations was about 80% viral and 20% cellular as judged by equilibrium centrifugation in cesium chloride density gradients.
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机译：在大型滚瓶中的WI-38细胞中产生了高滴度的人巨细胞病毒（CMV）品系AD 169。感染后第4天观察到最大噬菌斑滴度，此时培养基中的感染性是与细胞相关的200倍。通过在连续流离心机转子中以蔗糖梯度沉降来回收释放到培养基中的病毒。在蔗糖浓度为42％时发现最大的病毒感染力，相当于1.18 g / cm3的密度。从这些制剂中提取的脱氧核糖核酸在氯化铯密度梯度中通过平衡离心法判断为约80％的病毒和20％的细胞。
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