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首页> 外文期刊>Applied and Environmental Microbiology >Multilocus Sequence Typing and FlaA Sequencing Reveal the Genetic Stability of Campylobacter jejuni Enrichment during Coculture with Acanthamoeba polyphaga
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Multilocus Sequence Typing and FlaA Sequencing Reveal the Genetic Stability of Campylobacter jejuni Enrichment during Coculture with Acanthamoeba polyphaga

机译:多基因座序列分型和FlaA测序揭示了空肠弯曲杆菌富集与棘阿米棘芽孢杆菌共培养期间的遗传稳定性。

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摘要

Low concentrations of Campylobacter jejuni cells in environmental samples make them difficult to study with conventional culture methods. Here, we show that enrichment by amoeba cocultures works well with low-concentration samples and that this method can be combined with molecular techniques without loss of genetic specificity.
机译:环境样品中空肠弯曲杆菌细胞的浓度低,使其很难用常规培养方法进行研究。在这里,我们证明了通过变形虫共培养进行的富集对低浓度样品效果很好,并且该方法可以与分子技术结合使用而不会丧失遗传特异性。

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