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Improvement of the Representation of Bifidobacteria in Fecal Microbiota Metagenomic Libraries by Application of the cpn60 Universal Primer Cocktail

机译:通过应用cpn60通用引物鸡尾酒改善粪便微生物群基因组图书馆中双歧杆菌的表达

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摘要

Actinobacteria, particularly bifidobacteria, are widely observed to be underrepresented in metagenomic studies of microbial communities. We have compared human fecal microbiota clone libraries based on 16S rRNA and cpn60 PCR products. Taxonomic profiles were similar except that the cpn60 libraries contained large numbers of bifidobacterial sequences.
机译:广泛认为放线菌,特别是双歧杆菌在微生物群落的宏基因组学研究中代表性不足。我们已经比较了基于16S rRNA和 cpn60 PCR产物的人类粪便微生物克隆库。除 cpn60 文库包含大量双歧杆菌序列外,分类学特征相似。

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