Pathovars of Pseudomonas syringaeCausing Bacterial Brown Spot and Halo Blight in Phaseolus vulgaris L. Are Distinguishable by Ribotyping

摘要

Ribotyping was evaluated as a method to differentiate betweenPseudomonas syringae pv. phaseolicola and pv. syringae strains causing bacterial brown spot and halo blight diseases inPhaseolus vulgaris L. Ribotyping, with restriction enzymesBglI and SalI and using the Escherichia coli rrnB operon as the probe, differentiated 11 and 14 ribotypes, respectively, and a combination of data from both procedures yielded 19 combined ribotypes. Cluster analysis of the combined ribotypes differentiated the pathovars phaseolicola and syringae, as well as different clonal lineages within these pathovars. The potential of ribotyping to screen for correlations between lineages and factors such as geographical region and/or bean varieties is also reported.