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首页> 外文期刊>Applied and Environmental Microbiology >A Double-Selective Tissue Culture System for Isolation of Wild-Type Poliovirus from Sewage Applied in a Long-Term Environmental Surveillance
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A Double-Selective Tissue Culture System for Isolation of Wild-Type Poliovirus from Sewage Applied in a Long-Term Environmental Surveillance

机译:从污水中分离野生型脊髓灰质炎病毒的双重选择组织培养系统,在长期环境监测中的应用

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We describe a simple, cost-efficient, double-selective method for isolation of wild-type poliovirus from sewage samples containing vaccine polioviruses and other enteroviruses, with a detection limit of 18 to 50 PFU per 1 to 2 liters of sewage. By this method we were able to process 1,700 sewage samples collected between 1991 and 1996, from which 10,472 plaques were isolated, 41 of them being identified as wild-type polioviruses.
机译:我们描述了一种简单,经济高效的双选择方法,用于从含有疫苗脊髓灰质炎病毒和其他肠病毒的污水样品中分离野生型脊髓灰质炎病毒,每1至2升污水的检出限为18至50 PFU。通过这种方法,我们能够处理从1991年到1996年收集的1,700个污水样品,从中分离出10,472个噬菌斑,其中41个被鉴定为野生型脊髓灰质炎病毒。

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