首页> 外文期刊>Applied and Environmental Microbiology >Starved cells of the fatty acid auxotroph Escherichia coli AK7 develop abnormal sensitivity to media with low osmolarity.
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Starved cells of the fatty acid auxotroph Escherichia coli AK7 develop abnormal sensitivity to media with low osmolarity.

机译:脂肪酸营养缺陷型大肠杆菌AK7的饥饿细胞对低摩尔渗透压浓度的培养基产生异常敏感性。

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摘要

The unsaturated fatty acid auxotroph Escherichia coli AK7 supplied with linolenic acid, while appearing normal during logarithmic growth, showed a fast decline in CFU during starvation as a result of an osmotic downshift when transferred to standard agar plates unsupplemented with an osmolyte such as 300 mosM sucrose or salt (NaCl or KCl). The starved cells could recover their osmoresistance when an energy source was added to the starvation medium.
机译:亚麻酸提供的不饱和脂肪酸营养缺陷型大肠埃希氏菌AK7在对数生长过程中表现正常,但在饥饿期间,由于渗透率下降,当转移到未添加渗透剂如300 mosM蔗糖的标准琼脂平板时,CFU迅速下降或盐(NaCl或KCl)。当将能量源添加到饥饿培养基中时,饥饿的细胞可以恢复渗透压。

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