Effects of Hemolysis on the Roche Ammonia Method for Hitachi Analyzers

摘要

The manufacturer’s product literature for the Roche ammonia reagent (cat. no. 11877984) states that “there is no interference from hemolysis up to a hemolysis index of 50 (approximate hemoglobin concentration: 50 mg/dL)”. This statement can and has been interpreted to mean there are artifactual changes in the ammonia results above this hemolysis index limit. The likely result is that laboratories could use this low limit as the definitive guide and not report any results above it. This would lead to rejection of up to 10% of samples.The effect of hemolysis on analytes is often studied [e.g., Ref. (1 …