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Comparative transcriptomic analysis reveals gene expression associated with cold adaptation in the tea plant Camellia sinensis

机译:比较转录组学分析揭示了茶树茶中与冷适应相关的基因表达

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Low temperature restricts the planting range of all crops, but cold acclimation induces adaption to cold stress in many plants. Camellia sinensis, a perennial evergreen tree that is the source of tea, is mainly grown in warm areas. Camellia sinensis var. sinensis (CSS) has greater cold tolerance than Camellia sinensis var. assamica (CSA). To gain deep insight into the molecular mechanisms underlying cold adaptation, we investigated the physiological responses and transcriptome profiles by RNA-Seq in two tea varieties, cold resistant SCZ (classified as CSS) and cold susceptible YH9 (classified as CSA), during cold acclimation. Under freezing stress, lower relative electrical conductivity and higher chlorophyll fluorescence (Fv/Fm) values were detected in SCZ than in YH9 when subjected to freezing acclimation. During cold treatment, 6072 and 7749 DEGs were observed for SCZ and YH9, respectively. A total of 978 DEGs were common for both SCZ and YH9 during the entire cold acclimation process. DEGs were enriched in pathways of photosynthesis, hormone signal transduction, and transcriptional regulation of plant-pathogen interactions. Further analyses indicated that decreased expression of Lhca2 and higher expression of SnRK2.8 are correlated with cold tolerance in SCZ. Compared with CSA, CSS was significantly more resistant to freezing after cold acclimation, and this increased resistance was associated with an earlier expression of cold-induced genes. Because the greater transcriptional differentiation during cold acclimation in SCZ may contribute to its greater cold tolerance, our studies identify specific genes involved in photoinhibition, ABA signal conduction, and plant immunity that should be studied for understanding the processes involved in cold tolerance. Marker-assisted breeding focused on the allelic variation at these loci provides an avenue for the possible generation of CSA cultivars that have CSS-level cold tolerance.
机译:低温限制了所有农作物的播种范围,但是冷驯化使许多植物适应冷胁迫。茶树是多年生常绿树,是茶的来源,主要生长在温暖的地区。茶花中华(CSS)比山茶具有更大的耐寒性。阿萨米卡(CSA)。为了深入了解冷适应的分子机制,我们在冷驯化过程中通过RNA-Seq调查了两种茶品种的生理响应和转录组谱,耐寒SCZ(分类为CSS)和易感性YH9(分类为CSA) 。在冰冻胁迫下,进行冷冻驯化时,与ZH9相比,在SCZ中检测到较低的相对电导率和较高的叶绿素荧光(Fv / Fm)值。在冷处理期间,观察到SCZ和YH9分别为6072和7749 DEG。在整个冷驯化过程中,SCZ和YH9共有978个DEG。 DEG富含光合作用,激素信号转导和植物-病原体相互作用的转录调控途径。进一步的分析表明,Lhca2的减少表达和SnRK2.8的较高表达与SCZ的耐寒性有关。与CSA相比,CSS在冷驯化后对冻结的抵抗力明显增强,并且这种抵抗力的增强与寒冷诱导基因的早期表达有关。因为在SCZ的冷适应过程中更大的转录分化可能有助于其更大的耐寒性,所以我们的研究确定了与光抑制,ABA信号传导和植物免疫有关的特定基因,应该对其进行研究以了解与耐寒性有关的过程。集中在这些基因座上的等位基因变异的标记辅助育种为可能产生具有CSS级耐寒性的CSA品种提供了一条途径。

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