Basal Transcription Factor 3 Plays an Important Role in Seed Germination and Seedling Growth of Rice

摘要

BTF3 has been recognized to be involved in plant growth and development. But its function remains mostly unknown during seed germination and seedling stage. Here, we have analyzed OsBTF3-related sequences inOryza sativaL. subspecies, japonica, which resembles with the conserved domain of a nascent polypeptide associated complex (NAC) with different homologs of OsBTF3 and human BTF3. Inhibition ofOsj10gBTF3has led to considerable morphological changes during seed germination and seedling growth. Germination percentage was not influenced by the application of GA3, ABA, and NaCl but all concentrations caused wild-type (WT) seeds to germinate more rapidly than the RNAi (Osj10gBTF3Ri) transgenic lines. Seedling inhibition was more severe in theOsj10gBTF3Riseedlings compared with their WT especially when treated with 100 or 200 μM GA3; 50% reduction in shoots was observed inOsj10gBTF3Riseedlings. The expression ofOsj3g1BTF3,Osj3g2BTF3andOsj10gBTF3was primarily constitutive and generally modulated by NaCl, ABA, and GA3stresses in bothOsj10gBTF3Rilines and WT at the early seedling stage, suggesting thatOsj3g1BTF3andOsj10gBTF3are much similar but different fromOsj3g2BTF3in biological function. These results show that OsBTF3 plays an important role in seed germination and seedling growth gives a new perception demonstrating that more multifaceted regulatory functions are linked with BTF3 in plants.