THE EFFECT OF CLPS AND RYR1 GENE POLYMORPHISM ON MEAT QUALITY OF Z?OTNICKA SPOTTED PIGS

摘要

The aim of the study was to relate the polymorphisms at CLPS and RYR1 genes to pork quality in case of Z?otnicka Spotted breed. The experiment was carried out on 30 purebred Z?otnicka Spotted pigs, which were reared in standard conditions and slaughtered at approximately 100 to 110 kg body weight. Genetic polymorphisms were determined with the PCR-RFLP procedure. Polymorphism at the colipase gene (CLPS) was identified with DdeI restriction enzyme and stress sensibility gene (RYR1) with HinPI. In the studied pig sample three genotypes at locus CLPS/DdeI and two genotypes at locus RYR1/HinPI were found. The genotype frequencies at CLPS gene were 0.07 for AA, 0.40 for AB and 0.53 for BB. Colipase gene affected intramuscular fat content in RYR1/CT pigs. Meat from pigs with genotype BB had higher intramuscular fat content (2.50%) compared to AA group (1.59%); P≤0.05. Moreover, significant influence of gene RYR1T on pH1 trait was shown. Values of all analyzed traits were within ranges characterizing good meat quality of the native Polish Z?otnicka Spotted pig.