Fourier transform infrared spectroscopy analysis of the active components in serum of rats treated with Zuogui Pill

摘要

Objective To investigate the active components Zuogui Pill, a typical recipe for nourishing kidney essence in the traditional Chinese medicine. Methods Adult male Sprague Dawley rats were treated with the traditional Chinese herbal medicine Zuogui Pill and the active components found in the serum of the animals were analyzed by Fourier transform infrared (FTIR) spectroscopy. FTIR spectra of serum samples of treated and untreated rats were analyzed and the A 2960 /A 2931 and A 1540 /A 1080 ratios were calculated. Results A 2960 /A 2931 ratios of the serum samples collected following the administration of Zuogui Pill were significantly higher than those of the normal serum samples. FTIR data were then fitted using a Gaussian equation for wave numbers in the range of 1140–1000?cm ?1 . A RNA /A DNA ratios in the serum of rats treated with Zuogui Pill were higher than those found in normal rat serum. Conclusion FTIR spectroscopy could be used as an analytical tool to detect the active components in serum of animals treated with Zuogui Pill.