Reproductive Characteristic of a Precocious Line of E.Tenella Sporozoite as Material Bioactive in Embyionating Eggs and the Implications of the Findings Appraised

摘要

The reproductive characteristics of a precocious line of E. tenella sporozoite were investigated in chicken embryos and the implications of the findings appraised. While it was possible to reproduce E. tenella in chicken embryos, other species could not be propagated efficiently. The results of this experiments showed that the reproductive index of this line of E. tenella in chickens, the propagation in eggs is presently more efficient and yields are variable. There was considerable variation in the oocyst yields among experiments, although the yields within an experiment among individual embryos did not vary greatly. It should be possible to achieve higher oocyst yields and enhanced embryo-adaptation (leading to decreased embryonic mortality) through increased serial passage in eggs, thereby maintaining immunogenicity in the vaccinated host.