Evaluation of Seed Germination Inhibition Test for Early Detection of Pregnancy in Cross Breed Dairy Cattle in Bangladesh

摘要

This piece of study was aimed to evaluate the seed germination inhibition test to diagnose pregnancy in cross breed dairy cattle in Bangladesh Agricultural University Dairy Farm, Mymensingh. The urine samples collected from fifty crossbred cows and heifers early in the morning on day 14, 21, 28, 35 and 45 after artificial insemination (AI) were subjected to seed germination test using wheat seeds. In each sterile petridish, fifteen (15) wheat seeds were taken on the filter paper and 15 ml of diluted urine was added (Ratio of urine and distilled water is1:4). The seed germination inhibition percentages were observed after three days of sampling whereas shoot length growth inhibition was observed after five days. Germination inhibition percentage of AI cows, significantly higher compare to non AI cows and water control groups at day 28 (p < 0.01), 35 (p < 0.001), and 45 (p <0.001) respectively. On the other hand shoot length growth of AI cows, significantly higher compare to non AI cows and water control groups at day 28 (p < 0.001), 35 (p< 0.001 and 45 (p <0.001) respectively . So, the increased seed germination inhibition percentage and reduced shoot length in post AI cross bred cattle was indicative of pregnancy state and from this study it is concluded that the seed germination inhibition techniques useful to detect pregnancy in cross bred cattle as a simple and economical method and it may be applied at the field level in Bangladesh.