Seed germination inhibition test for pregnancy detection in Malnad Gidda cows?-

摘要

In the present study, the seed germination inhibition technique was applied to diagnose pregnancyin Malnad Gidda cattle breed, a dwarf breed found in coastal and neighboring heavy rainfall areas inKarnataka.The urine samples collected from six inseminated Malnad Gidda cows at two months ofpost insemination served as positive group and the urine from six non inseminated Malnad Giddacows served as negative group. In both the cases, the urine was diluted at the ratio of 1:4 withdistilled water.In each sterile Petri dish fifteen wheat seeds were taken on the blotting paper and 15ml of diluted urine was added. For each cow the test was conducted with a replica of six tests in sixPetri dishes.Control test was also carried out with the addition of water only to the wheat seeds.ThePetri dishes were covered with the trays to avoid evaporation. After three days, the seeds wereexamined for germination inhibition percentage in positive, negative and control groups, whereinthe mean germination inhibition percentage was 73.65 ± 2.81, 27.90 ± 2.56 and 21.48 ± 2.69,respectively.The mean shoot length of the germinated wheat seeds on fifth day was 0.95 ± 0.47,3.62 ± 0.51 and 5.54 ± 0.68 cm in positive, negative and control groups, respectively. Meangermination inhibition percent and reduced shoot length in positive group of Malnad Gidda cattlewas indicative of pregnancy state. It was concluded that the seed germination inhibition techniqueis useful to detect pregnancy in Malnad Gidda cattle as a simple, non-invasive and economicalmethod.