Variation in pre-extraction processes influences the differences in chemical constituent, quantity and biochemical activities of volatile oils from Crinum jagus (Th.) D.: Gas Chromatography-Mass Spectrometry analysis

摘要

A comparative study performed on essential oil composition, cytotoxic and antioxidant activitiesof fresh and dried samples involving leaf and bulb of Crinum jagus (Th.) D. were reported.Qualitative and quantitative variations in the composition of oils were investigated using GCand GC-MS. The oil extracted from the leaf of C. jagus’s was characterized by high proportionsof beta-ocimene (10.0–13.8%), followed by hexadecane (2.6–11.1%), tetramethylpentadecane(9.3–10.4%) and phytol (7.0–9.0%). Bulb oil is rich in 14-methylpentanedecanoic acid methylester (20.6–22.5%), tetratetracontane (7.9–10.0%) and 9,12-octadecadinenoic acid (14.0–14.2%).Dried leaves and dried bulbs exhibited the highest cytotoxic activity (IC50 of 0.002 and 0.003μg/mL, respectively) followed by fresh leaves 0.033 μg/mL and fresh bulb 0.55 μg/mL. The essentialoils at 1.0 mg/mL displayed significant antioxidant activities. The level of antioxidant capacityvaried according to samples. The chemical constituents and quantities significantly varied basedon sample pre-extraction processing, thus affecting the biochemical activities.