Validation of a LC-MS/MS Multiresidue Methodology Based on a QuEChERS Approach for the Determination of Fluoroquinolones, Sulfonamides and Trimethoprim in Poultry and Porcine Kidney According to the Normative Instruction 24/2009-MAPA

摘要

This work involved the validation of a multiresidue method according to the Normative Instruction 24/2009-MAPA for determining 25 analytes, among fluoroquinolones, sulfonamides and trimethoprim in samples of poultry and porcine kidney. The extraction procedure was based on a QuEChERS approach. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed using the selected reaction monitoring mode (SRM) and ESI + ionization. All of the validated figures of merit were evaluated as satisfactory. Accuracy was assessed by recovery studies, varying from 82.7 to 115.5% for porcine kidney and from 91.5 to 110.4% for poultry kidney. Relative standard deviations were lower than 25.5% for porcine kidney, and 29.8% for poultry kidney. Decision limits (CC?±) comprised values from 10.37 to 3298.43 ?μg kg -1 for porcine kidney and 10.08 to 3176.59 ?μg kg -1 for poultry kidney. Detection capabilities (CC?2) varied from 10.73 to 3396.86 ?μg kg -1 for porcine kidney and 10.67 to 3253.19 ?μg kg -1 for poultry kidney. The developed method has been successfully employed in the routine analysis of incurred samples.